Professor

Research Director, CNRS
Emeritus Professor, The University of Tokyo
Head of Health and Environment Dpt, Yncrea Hauts de France 

SMMiL-E project
LIMMS/CNRS-IIS the University of Tokyo, UMI 2820

Contact: collard@iis.u-tokyo.ac.jp


RESEARCH INTERESTS

  • BIOMEMS and Cancer
  • Microsystems technologies, actuators and sensors
  • Cell biomechanical and bioelectrical measurements, simulation and analysis

BIOGRAPHY

Dominique Collard (IEEE M’01) was born in Cambrai, France in 1958. He received the Eng. Degree from ISEN (Institut Supérieur d’Electronique et du Numérique) in 1980, and the PhD degree from the University of Lille in 1984. From 1985 to 1986, he was with the Toshiba VLSI Research Center, Kawasaki, Japan, as a Visiting Scientist. Since 1988, he has been with the Centre National de la Recherche Scientifique (CNRS), being alternatively with the Institut d’Electronique, de Microélectronique et de Nanotechnologie (IEMN), Lille, and with the Laboratory for Integrated MicroMechatronic Systems (LIMMS/CNRS-IIS), Tokyo. From August 2005, he joint a second time LIMMS where he was appointed Director in Sept 2007 and got a title of Project Professor of IIS, the University of Tokyo. In Dec. 2011, he became coordinator of EC/FP7 INCOLAB: EUJO-LIMMS aiming to open LIMMS to European partners and first EC laboratory in Japan. His current scientific interest covers micro and nano systems for applications in biology and nanotechnology. He is authors or co-author of more than 300 international publications. Dominique Collard got the IBM price on intensive numerical calculation in 1990, the CNRS bronze medal in 1992, and was in 2004 Laureate of the French academic palms from Ministry of higher education and research. In June 2014 he was named as Director the SMMiL-E Project (Seeding Micro System in Medecine in Lille: European-Japanese technologies against Cancer) to pull BioMEMS to Cancer research of Lille, France in a project supported by CNRS, IIS/The University of Tokyo, Lille-1 University, Centre Oscar Lambret. In April 2016, I joint the Lille Site of LIMMS to develop the SMMiL-E project and to manage the State Region plan toward the creation of a new interdisciplinary research against Cancer.


EDUCATION

  • 1994 Habilitation for Research Direction: Doctoral School, University of Lille, Lille, France
  • 1984 PhD, Electrical Engineering, Doctoral School, University of Lille, Lille, France
  • 1980 Advanced study degree (DEA) in material physics, University of Lille, Lille, France
  • 1980 Engineer degree from Institut Supérieur d’Electronique du Nord (ISEN), Lille, France

PROFESSIONAL ACTIVITIES

  • 2015-present Head of Health and Environment Dpt, Yncrea Hauts de France, Lille, France
  • 2014-present Coordinator of the Region Plan against Cancer in Hauts de France, France
  • 2014-2018 Director of SMMiL-E project, LIMMS/CNRS-IIS, The University of Tokyo
  • 2005-2015 Director of LIMMS, LIMMS/CNRS-IIS, The University of Tokyo, Japan
  • 1997-2005 MEMS group Leader, IEMN UMR CNRS, Lille, France
  • 1997-present Director of Research CNRS
  • 1995-1997 Director of LIMMS, LIMMS/CNRS-IIS, The University of Tokyo, Japan
  • 1988 – 1997 Researcher CNRS
  • 1985-1986 Invited scientific researcher TOSHIBA, Kawasaki, Japan

HONORS AND AWARDS

  • 2019 Emeritus Professor, The University of Tokyo, Japan
  • 2004 French academic palms from Ministry of higher education and research
  • 2000 Advisor at the French Ministry of Research and Technology (Dir. of Technology)
  • 1990 CNRS Bronze medal 1992
  • 1990 Co-laureate of IBM price on intensive numerical calculation

HIGHLIGHTED PAPERS 

  • 2017 Highlighted paper in Small (Cover)
  • 2015 Selected Paper in Transducers 2015, Anchorage, USA
  • 2014 Highlighted Paper in Lab. On. Chip. (Internal cover)
  • 2011 Highlighted Paper in J. Micromech. Microeng. (2011 selection)
  • 2008 Highlighted Paper in Chem. Phys. Chem. (Cover)

SELECTED ONGOING PROJECTS

  • Real-time mechanical characterization of DNA degradation under therapeutic X-rays
    The killing of tumor cells by ionizing radiation beams in cancer radiotherapy is currently based on a rather empirical understanding of the basic mechanisms and effectiveness of DNA damage by radiation. By contrast, the mechanical behaviour of DNA encompassing sequence sensitivity and elastic transitions to plastic responses is much better understood. A novel approach is proposed based on a micromechanical Silicon Nanotweezers device. This instrument allows the detailed biomechanical characterization of a DNA bundle exposed to an ionizing radiation beam delivered here by a therapeutic linear particle accelerator (LINAC). The micromechanical device endures the harsh environment of radiation beams and still retains molecular-level detection accuracy. In this study, the first real-time observation of DNA damage by ionizing radiation is demonstrated. The DNA bundle degradation is detected by the micromechanical device as a reduction of the bundle stiffness, and a theoretical model provides an interpretation of the results. These first real-time observations pave the way for both fundamental and clinical studies of DNA degradation mechanisms under ionizing radiation for improved tumour treatment.
  • Distinguishing cancer cells based on their biophysical signatures
    Changes in cell shape and structural integrity affect many biological processes related to cells. Therefore, we can potentially use the biophysical properties of cells to reflect the state of their health. This connection between the biophysics and diseases has been attracting scientific research attention, especially for cancer research, where diseased cells proliferate uncontrollably and disrupt the organization of tissue. Here, we target a reliable and practical high-throughput technique to obtain the biophysical signature of cancer cells. We take two parallel approaches to achieve this goal. We use MEMS grippers (i.e., Silicon NanoTweezers) that provide higher sensitivity to examine different biophysical properties (e.g., size, stiffness, viscosity, and electrical properties). In parallel, we are developing a high-throughput MEMS device optimized according to the SNT results for clinical applications.
  • Real-time measurement of the physical properties of DNA-ligand complexes
    We are using a MEMS gripper device, Silicon Nanotweezers (SNT), to monitor the stiffness of λ-phage DNA through increasing concentrations of ligands (such as Doxorubicin (a DNA binding drug widely used in chemotherapy), SybrGreen and Hoechst) in real-time. The study of the mechanical properties of DNA-ligand complexes can provide valuable insight on the biological implications of such complexes in-vivo.

SELECTED PUBLICATIONS

  • S. Kaneda, J. Kawada, M. Shinohara, M. Kumemura, R. Ueno, T. Kawamoto et al. Boyden chamber-based compartmentalized tumor spheroid culture system to implement localized anticancer drug treatment. Biomicrofluidics. Vol. 13, p. 054111, 2019.
  • Y. Tauran, M. Kumemura, M. C. Tarhan, G. Perret, F. Perret, L. Jalabert, et al., Direct measurement of the mechanical properties of a chromatin analog and the epigenetic effects of para-sulphonato-calix[4]arene, Scientific Reports, vol. 9, p 5816, 2019.
  • Y. Takayama, G. Perret, M. Kumemura, M. Ataka, S. Meignan, S Karsten, et al. Developing a MEMS Device with Built-in Microfluidics for Biophysical Single Cell Characterization. Micromachines. Vol. 9, pp 275, 2018.
  • C. M. Tarhan, R. Yokokawa, L. Jalabert, D. Collard, and H. Fujita, Pick-and-Place Assembly of Single Microtubules, Small, vol. 13, p. 1701136, 2017.
  • S. L. Karsten, M. Kumemura, L. Jalabert, N. Lafitte, L. C. Kudo, D. Collard, et al., Direct electrical and mechanical characterization of in situ generated DNA between the tips of silicon nanotweezers (SNT), Lab on a Chip, vol. 16, pp. 2099-2107, 2016.
  • G. Perret, T. Lacornerie, F. Manca, S. Giordano, M. Kumemura, N. Lafitte, et al., Real-time mechanical characterization of DNA degradation under therapeutic X-rays and its theoretical modeling, Nature.com/Microsystems & Nanoengineering, vol. 2, p. 16062, 12/05/online 2016.
  • D. Collard, S. H. Kim, T. Osaki, M. Kumemura, B. Kim, D. Fourmy, T. Fujii, S. Takeuchi, S. L. Karsten, and H. Fujita, “Nano bioresearch approach by microtechnology”, Drug discovery today, vol. 18, pp. 552-559, 2013