PhD student 

Supervisors: Fabrizio CLERI and Mehmet Cagatay Tarhan

LIMMS/CNRS-IIS, The University of Tokyo, Lille, France
SMMiL-E project
BioMEMS group, IEMN, Lille, France


Contact: quentin.rezard.etu@univ-lille.fr


EDUCATION

  • 2019 to now Ph. D. studies
    • IEMN, CNRS, Villeneuve d’Ascq
  • 2017-2018    Master 2 degree in Complex Fluids and divided media
    • Université Paris-Sud, Université Paris Saclay, Orsay
  • 2016-2017    Master 1 in Physics and Applications course Condensed Matter and Nanosciences
    • Université Paris-Sud, Université Paris Saclay, Orsay
  • 2012-2016    Bachelor’s degree in Physics and Application
    • Université Paris-Sud, Université Paris Saclay, Orsay
  • 2010-2012    Common first year of health studies
    • Université Paris-Sud

PROFESSIONAL ACTIVITIES

  • 2018 – R&D Interneship, Microfluidics, MEMS et nanostructures laboratory, Gulliver, Institut Pierre-Gilles de Gennes, Paris, France
  • 2017  Lab Internship, Laboratoire de Chimie Physique, Université Paris-Sud, CNRS, Orsay, France 

SELECTED ONGOING PROJECTS

  • High-throughput identification of circulating tumor cells using their biophysical signature
    Due to their scarce concentration and heterogeneity, circulating tumor cells (CTCs) are difficult to detect. We need a fast, reliable and practical method to include them in routine medical examinations. Using biophysical markers, we introduced a MEMS device to perform direct measurements on electrical and mechanical properties of single cancer cells (Figure). Working in a continuous flow format permits high-throughput measurements. The device design allows us to define fixed or movable electrodes as part of the side-walls of an embedded microchannel. Moreover, the movable electrode is in direct contact with passing cells which enables direct sensing during compression. The device can perform both electrical and mechanical measurements on single cells in a continuous flow targeting biophysical parameters as cell size, membrane capacitance, cytoplasm resistivity and stiffness. The proposed method aims at providing high-throughput biophysical cytometry, in an optics- and marker-free way, for cancer cell evaluation. 
Figure: a) A general SEM view of the proposed device. The functional part of the device consists of 3 parts:
(i) a microfluidic channel embedded in the top layer of an SOI wafer to handle cells, (ii) an electrical detection area to perform electrical measurements in continuous flow and (iii) a mechanical detection area to stimulate cells mechanically during simultaneous measurements. b) The working principle shows how cells are first characterized electrically and then mechanically in a continuous flow.

SELECTED PUBLICATIONS

  • Q. Rezard, G. Perret, J.C. Gerbedoen, D. Pekin, F. Cleri, D. Collard, C. Lagadec and M. C. Tarhan, “Developing a MEMS device for high-throughput multi-parameter single cell biophysical analysis”, IEEE International Conference on Micro Electro Mechanical Systems (MEMS’21), pp. 494, 2021.
  • Q. Rezard, G. Perret, J.C. Gerbedoen, D. Pekin, D. Collard, C. Lagadec and M. C. Tarhan, “A high-throughput MEMS device for mechanical detection of cancer cells”, 24rdInternational Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS’20), pp. 805, 2020.
  • D. Pekin, G. Perret, Q. Rezard, J.C. Gerbedoen, D. Collard, C. Lagadec and M. C. Tarhan, “Subcellular imaging during single cell mechanical characterization”, IEEE International Conference on Micro Electro Mechanical Systems (MEMS’20), pp. 62, Vancouver, Canada, 2020.
  • M. Pascual, M. Kerdraon, Q. Rezard, M.-C. Jullien and L. Champougny, “Wettability patterning in microfluidic devices using thermally-enhanced hydrophobic recovery of PDMS” Soft Matter, 2019,15, 9253-9260